Connexin 43 hemichannels mediate the Ca2+ influx induced by extracellular alkalinization
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چکیده
منابع مشابه
Connexin 43 hemichannels mediate the Ca2+ influx induced by extracellular alkalinization.
Although alkaline pH is known to trigger Ca(2+) influx in diverse cells, no pH-sensitive Ca(2+) channel has been identified. Here, we report that extracellular alkalinization induces opening of connexin 43 hemichannels (Cx43 HCs). Increasing extracellular pH from 7.4 to 8.5, in the presence of physiological Ca(2+)/Mg(2+) concentrations, rapidly increased the ethidium uptake rate and open probab...
متن کاملConnexin 43 hemichannels mediate the Ca influx induced by extracellular alkalinization
Kurt A. Schalper, Helmuth A. Sánchez, Sung C. Lee, Guillermo A. Altenberg, Michael H. Nathanson, and Juan C. Sáez Departamento de Ciencias Fisiológicas, Pontificia Universidad Católica de Chile, Santiago, Chile; Department of Cell Physiology and Molecular Biophysics, and Center for Membrane Protein Research, Texas Tech Health Sciences Center, Lubbock Texas; Department of Internal Medicine, Yale...
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Wistar rat pups had the left common carotid artery cut, and they were exposed to 8% oxygen with free access to food and water until they were killed at 1, 12, 24, and 48 hours after the hypoxia-ischemia (HI) insult. Connexin 43 (Cx43), hemichannel (HC1), and caspase 3 (Casp3) in cerebral HI tissues were examined by immunohistochemistry and Western blot analyses. Astrocytes cell line, astrocytes...
متن کاملConnexin 43 hemichannels are permeable to ATP.
Astrocytes are electrically nonexcitable cells that communicate by means of Ca(2+) signaling. Long-distance intercellular Ca(2+) waves are initiated by release of ATP and activation of purinergic receptors on nearby cells. Previous studies have implicated connexin 43 (Cx43) in ATP release, but definitive proof that ATP exits through Cx43 hemichannels does not exist. Here, through several altern...
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Cell function and survival are controlled by intracellular signals, and modulated by surrounding cells and the extracellular environment. Connexin channels participate in these processes by mediating cell-to-cell communication. In bone cells, gap junction channels were detected in the early 1970s, and are present among bone resorbing osteoclasts, bone forming osteoblasts, and osteocytes - matur...
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ژورنال
عنوان ژورنال: American Journal of Physiology-Cell Physiology
سال: 2010
ISSN: 0363-6143,1522-1563
DOI: 10.1152/ajpcell.00015.2010